Molecular Biology
GoodView Nucleic Acid Stain
GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and the other at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm.
- SKU: RBHGV-2
- CATEGORY: Molecular Biology
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Cat. No.: RBHGV-2
Comparative sensitivity test of GV and EB
- Sensitivity test of GoodViewTM Nucleic Acid Stain
- Template：5～70 ng λDNA（λDNA diluted to different concentrations）
- GoodView：add 5 μl GoodView to 100 ml 1% agarose gel solution.
- Sensitivity test of EB
- Template：5～70 ng λDNA（λDNA diluted to different concentrations）
- EB：add 5 μl EB (10 mg/ml) to 100 ml 1% agarose gel solution, to make the final concentration to 0.5 ng/μl.
The result of electrophoresis demonstrates GV is almost as sensitive as EB.
Conclusion:
- When comparing Fig.1 and Fig.2, it is clearly demonstrated that GoodViewTM has a much better sensitivity than product A.
- The difference between Fig.3 and Fig.4 illustrates that although product B has a good sensitivity and a low background, it has shortage: apparently the ladder cannot be distinctively separated.
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