The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDSPAGE) with Tris-glycine-SDS running buffer. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heats, dilute, and add reducing agent before loading. Features   Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer)   Ready-to-use: supplied in a loading buffer for direct loading on gels   Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green      and a red dye   Sharp bands Applications   Monitoring of protein migration during SDS-PAGE.   Monitoring of protein transfer onto membranes during Western blots.   Sizing of proteins on SDS-PAGE and Western blots. Storage Buffer Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Quality Control The quality of the BLUelf Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality. Storage Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C. BLUelf Prestained Protein Ladder Protocol 1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to     dissolve precipitated solids. Do not boil. 2. Mix thoroughly to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on SDS-PAGE gel:   15 μl per well for mini-gels, 2.5 μl per well for blots   10 μl per well for large gels, 5 μl per well for blots   Apply more for thicker (> 1.5 mm) or larger gel Guide for Molecular Weight Estimation (kDa) Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis conditions are listed below:GeneDireX, Inc.www.genedirex.com info@genedirex.com